Accessing FITC in the terminal of the whole chain, and before this accessing a molecule of Acp (6-aminocaproic acid), also known as alkyl spacer
Accessing FITC to a side chain of Lys in the whole peptide. Lys side chain’s end -NH2 is four carbon alkyl, which directly reduces the steric effect
| Fluores cent | dansyl chloriade | Hydroxy-coumarin | AMC | Methoxy coumarin | ALexar fluor | aminocou-marin | Dabcyl | Cy2 | FAM | Alexa fluor488 | FITC | Alexa fluor430 | 5-FAM | Alexa fluor532 | HEX | 5-TAMRA | Cy3 | TRITC |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ex(nm) | 325 | 340 | 345 | 360 | 345 | 350 | 453 | 490 | 495 | 494 | 495 | 430 | 492 | 530 | 535 | 542 | 550 | 547 |
| Ex(nm) | 386 | 578 | 445 | 410 | 442 | 445 | - | 510 | 517 | 517 | 519 | 545 | 518 | 555 | 556 | 568 | 570 | 572 |
| Fluores cent | R-phycoery-thrin(PE)(489) | RhodamineRed-X | Tanara | ALexar fluor555 | ALexar fluor546 | Rox | ALexar fluor568 | Texa Red | ALexar fluor594 | Alexa fluor | Alexa fluor633 | Cy5(625) | Alexa fluor660 | Cy5.5 | Tru Red | Alexa fluor680 | Cy7 | Cy3.5 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ex(nm) | 565 | 560 | 560 | 565 | 556 | 575 | 578 | 589 | 590 | 621 | 650 | 650 | 663 | 675 | 675 | 679 | 743 | 581 |
| Ex(nm) | 578 | 580 | 580 | 573 | 573 | 602 | 603 | 615 | 617 | 639 | 668 | 670 | 690 | 694 | 695 | 702 | 767 | 596 |
For long-term storage, peptides should be stored in lyophilized form at -20℃, or preferably at -80℃ with desiccant in sealed containers to minimize peptide degradation. Fluorescent Peptides also should be kept away from light.
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MD2 mediates angiotensin II-induced cardiac inflammation and remodeling via directly binding
To Ang II and activating TLR4/NF-κB signaling pathway,Basic Research in Cardiology,Weijian Huang.
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